Results of a study of the effectiveness of direct coronary oxygen persufflation as a donor heart conditioning method

Objective: to evaluate the technical feasibility as well functional, metabolic and structural integrity of donor heart myocardium after 4 hours direct intracoronary oxygen persufflation in an experiment. Materials methods. Mini-pig siblings aged 3 months with a body weight 23–36 kg were used experimental model. In control group (n = 8), hearts cold preserved by injecting 2 liters Bretschneider cardioplegic solution (Custodiol®, Germany, HTK) into aortic root. modified HTK (with 40 mg/L hyaluronidase added) was initiate cardioplegia, then moistened carbogen (95% O , 5% CO ) injected ascending aorta, maintaining 40–45 mm Hg root pressure. The stored mHTK at 0–4 °С. After preservation, orthotopic transplantation (OHTx) performed. post-transplant period, we studied central hemodynamic parameters, myocardial consumption, level ischemia markers (troponin I, TnI; creatine phosphokinase-MB, CPKMB; lactate dehydrogenase, LDH), histological signs cellular injury. Results. Sixteen OHTx surgeries performed during study. At 120 minutes restoration spontaneous cardiac activity, output 2.99 [4.85; 3.17] L/min 2.48 [2.04; 2.92] (p > 0.05) groups, respectively. Changes LDH, TnI levels blood flowing from coronary sinus significantly higher early reperfusion period. However, there no statistically significant difference between groups 0.05). Myocardial consumption 8.2 [7.35; 9.35] ml-O2/min/100 g 7.7 [6.75; 10.12] ml-O /min/100 g, respectively Morphological examinations also showed injury compared group. Conclusion. experiment safety for ex vivo conditioning stage. same time, data advantages over standard protocol preservation solution.